Hospitalisation Area. Located in Unit 36 (Floor 6 of Building 3); access via Plaza de Cristo Rey and taking lifts 1, 2 and 3.

It has the following resources:

  • Conventional hospital beds (as needed).
  • 6 isolation beds with HEPA (High Efficiency Particle Arresting) filters for Haematopoietic Progenitor Transplantation.
  • 1 medical office with videoconferencing system.
  • 2 nursing stations.

Outpatient Area. Located on Floor 1 of the hospital; access via Plaza de Cristo Rey and taking lifts 2 and 3.

It has the following resources:

  • 6 offices for consultations.
  • 1 waiting room.
  • 1 office for the Haematology/Oncology Department.
  • 1 Oncohaematology Day Hospital.

Bone Marrow Biopsy Area. Located on the Ground Floor of the hospital; access via Avenida Reyes Católicos Avenue towards the teaching area, or through the Research Building via the entrance to the Museum of America.

It has the following resources:

  • 1 bone marrow biopsy room.
  • 1 medical office.
  • 1 stainer for bone marrow extensions.
  • 2 microscopes.

Blood Bank and Haematopoietic Progenitor Transplant Laboratory Area. Located on the Ground Floor of the hospital; access via Avenida Reyes Católicos Avenue towards the teaching area, or through the Research Building via the entrance to the Museum of America.

It has the following resources:

  • 1 Blood Bank.
  • 1 Immunohaematology Laboratory.
  • 1 Haematopoietic Progenitor Transplantation and Cell Therapy Laboratory.
  • 1 aphaeresis room.
  • 2 medical offices.
  • 1 office for nursing supervisor.
  • 1 office for Blood Bank administration.
  • 1 blood donor room.
  • 1 waiting room.

Haematology and Diagnostic Haematology Laboratory Area. The Haematology and Diagnostic Haematology Laboratory area is in the basement, which can be accessed from the entrance located opposite the Museum of America.

It has the following resources:

  • Haemacytometry and peripheral blood cytology laboratory.
  • Erythropathology Laboratory.
  • Coagulation Laboratory.
  • Flow Cytometry Laboratory.
  • Haematological Molecular Cytogenetics Laboratory.

Technological equipment:

Blood Bank and Haematopoietic Progenitor Transplant Laboratory Area:

  • 2 vision autoanalysers (Ortho).
  • 3 refrigeration systems.
  • Laminar flow hood.
  • Liquid nitrogen tanks.
  • Cryopreservation equipment.
  • 2 COBE Optia aphaeresis systems.
  • 1 bag centrifuge.
  • Dry plasma thawer.
  • Minor equipment (centrifuges, microscopes).

Haematology and Haematology Diagnostic Laboratory Area:

  • Haematology analysis:
    • - State-of-the-art automatic counters with built-in digital data transmission: 6 Sysmex XN (Roche) connected in series with built-in automatic smear stainer and 2 VesCube systems.
    • Cytocentrifuges.
    • Cytochemistry.
    • Optical microscopes with the possibility of digital photography.
  • Coagulation:
    • 2 ACL TOP 700.
    • 1 ACL AcuStar (Werfen).
    • 1 aggregometer (Stago).
    • 1 PFA-100 (Siemens).
    • An automated analyser to measure ST-Genesia thrombin generation (Stago).
  • Cytometry:
    • 2 Multiparametric Flow Cytometers FacsCANTO II (8 colours).
    • 4 Analysis stations with Infinicyt program.
  • Molecular Biology:
    • 1 Abi Prism 7500 real-time PCR thermocycler (Applied Biosystems).
    • 1 RotorGene (QIAGEN).
    • 3 conventional thermocyclers.
    • 1 Gel Doc EZ Imager image analysis equipment (Bio-Rad).
    • 1 QIAgility automated pipetting device.
    • 1 Auto-LiPA PCR hybridiser/washer/revealer for PCR products.
    • 1 ABI-PRISM 3130 nucleic acid sequencer (Applied Biosystems) (located in the Genetics Department and shared with the Research Unit).
  • Molecular Cytogenetics:
    • An automated platform for metaphase scanning and capture (Metafer, Metasystems).
    • Four workstations equipped with Ikaros software (Metasytems) for karyotyping.
    • For the fluorescence in situ hybridisation (FISH) technique: two hybridisation plates (Leyca) and two fluorescence microscopes for fluorescence analysis.
    • For sequencing techniques: two SimpliAmp thermal cyclers (Applied Biosystems) and an ABIPRISM 3130 sequencer (Applied Biosystems) (IIS-FJD equipment).
    • For massive sequencing techniques: Illumina MiSeq (pathological anatomy service) and NextSeq (IIS-FJD team) sequencers.
    • Additional infrastructure in the Haematology Service:
  • Peripheral blood and bone marrow haematopoietic progenitor cell (HPC) collection laboratory.
  • Clinical programmes for HPC. HPC and bone marrow collection staff (physician and nurses). HPC and bone marrow collection accredited by JACIE.
  • Flow cytometry immunophenotyping laboratory. Molecular and cytogenetic laboratory. Cell culture room equipped with two laminar flow cabinets and two CO2 ovens. Two microscopes with Nikon camera for cell culture monitoring.
  • In January 2014, the IIS-FJD signed a 5-year agreement with CIEMAT to set up a Joint Unit for Advanced Therapies between the IIS-FJD and the Innovative Haematopoietic Therapies Division. It is still in force today, with active collaboration between FJD's haematology service and Dr Juan Bueren's group, offering general training in two main fields: stem cells and gene therapy. The new building has all the latest facilities for cell and gene therapy studies, including a new GMP facility that recently passed an inspection by the Spanish Medicines and Health Products Agency. This facility will be used to generate GMP 2G-MSC for clinical applications.

Techniques and procedures:


  • Bone marrow aspirates and bone marrow biopsies.
  • Abdominal fat punctures for diagnosis of amyloidosis.
  • Lumbar punctures and administration of intrathecal chemotherapy.
  • Plasmaphaeresis.
  • Stem cell aphaeresis for haematopoietic progenitor transplantation
  • Aphaeresis for cell therapy.


  • Diagnosis of red blood cell disorders (anaemias and polyglobulias), bone marrow failure, benign diseases of the white blood cell series, platelet disorders, acquired or congenital bleeding disorders, thrombosis and thrombophilia and disorders of iron metabolism (haemochromatosis).
  • Diagnosis of haematological malignancies, such as lymphomas, myelomas, myeloproliferative syndromes, myelodysplastic syndromes, chronic lymphocytic leukaemia and acute leukaemia.
  • Diagnosis of early and late complications related to Haematopoietic Progenitor Transplantation.
  • Long-term Cancer Survivors Programme. Early diagnosis and preventive medicine to avoid long-term complications following treatments for patients with haematological malignancies (leukaemia, lymphomas).
  • Haematogeriatrics Programme. Specialised care programme for older patients with haematological diseases, in order to diagnose special needs.
  • Control of oral anticoagulant treatment with dicoumarinic drugs (Sintrom®, Aldocumar®).
  • Health education on oral anticoagulants, both dicoumarinics and new oral anticoagulants.
  • Diagnosis of haematological and thrombotic pregnancy disorders.
  • Blood saving programme for surgery.
  • Studies prior to the administration of blood and blood derivatives.
  • Haemolytic disease of the foetus and newborn.


  • Administration of chemotherapy for haematological malignancies.
  • Administration of monoclonal antibodies.
  • Administration of molecular targeted therapy antineoplastics (tyrosine kinase inhibitors).
  • Conditioning for Haematopoietic Progenitor Transplantation.
  • Haematopoietic Progenitor Infusion.
  • Cell therapy (infusion of donor lymphocytes, mesenchymal cell therapy).
  • therapeutic phlebotomies.
  • Plasmaphaeresis.
  • Other therapeutic aphaeresis (erythroaphaeresis, leukaphaeresis).
  • Transfusion of blood components.
  • Intrauterine transfusions (in foetal haemolytic disease).
  • Transfusion of coagulation factors.
  • Administration with intravenous immunoglobulins.
  • Treatment with intravenous iron.